| 角色 | 演員 | 備註 |
| Spider-Man (24 episodes, 1981-1983) | Dan Gilvezan | |
| Angelica Jones (24 episodes, 1981-1983) | Kathy Garver | |
| Iceman (24 episodes, 1981-1983) | 弗蘭克·維爾克（Frank Welker） | |
| Narrator (15 episodes, 1981-1983) | 斯坦·李（Stan Lee） | |
| Opening announcer (13 episodes, 1981) | Dick Tufeld | |
| Aunt May Parker (8 episodes, 1981) | 瓊·弗瑞（June Foray） | |
| Captain America (3 episodes, 1981) | George DiCenzo | |
| Doctor Strange (3 episodes, 1981) | John Stephenson | |
| Red Skull (2 episodes, 1981) | Hans Conried | |
| Mona Osborn (2 episodes, 1981) | Sally Julian | |
| Dr. Faustus (2 episodes, 1981) | Dennis Marks | |
| Shanna the She-Devil (2 episodes, 1981) | Janet Waldo | |
| Katherine 'Kitty' Pryde (2 episodes, 1982-1983) | 梅麗莎·蘇·安德森（Melissa Sue Anderson） | |
| The Sandman (unknown episodes, 1981) | Chris Latta | |
| Juggernaut (unknown episodes, 1983) | William Marshall | |
| Dr. Octopus (unknown episodes) | Michael Bell | |
